Begin Mitochondria Repair / Enhancement 14 week protocol

WEEK 1-2 NAD+ LOADING PHASE

1. NAD+ 25mg twice per week

WEEK 3-8 SS-31 Mitochondrial Repair Phase

WEEK 3-4:

1. NAD+ 50mg twice per week
2. SS-31 4mg daily in morning M-F (4mg baseline 10mg for acute oxidative stress)
3. KLOW 1mg daily

WEEK 5-6:

1. NAD+ 100mg twice per week
2. SS-31 4mg daily in morning M-F (4mg baseline 10mg for acute oxidative stress)
3. KLOW 2mg daily

WEEK 6-8

1. NAD+ 100mg twice per week
2. SS-31 4mg daily in morning M-F (4mg baseline 10mg for acute oxidative stress)

WEEK 9-14 MOTS-c Metabolic Enhancement Phase

WEEK 9-10

1. NAD+ 100mg twice per week
2. MOTS-c 2 mg three times per week

WEEK 11-14

1. NAD+ 100mg twice per week
2. MOTS-c 5 mg three times per week (if tolerated or stick with 2)

Mechanisms & Rationale

SS‑31 (Elamipretide)

• Binds cardiolipin in the inner mitochondrial membrane.
• Stabilizes electron transport chain supercomplexes, reduces ROS, improves ATP production.
• Shown in animal studies to improve cardiac and muscle mitochondrial function.

MOTS‑c

• A mitochondrial‑encoded peptide that activates AMPK.
• Enhances glucose uptake, fatty‑acid oxidation, and stress resistance.
• Can increase NAD+ and activate SIRT1.
• Improves physical performance and metabolic health in animal studies.

NAD+ Supplementation

• Essential coenzyme for metabolism, DNA repair, and sirtuin activation.
• Levels decline with age; replenishment improves mitochondrial function and metabolic resilience.
• Dose escalation (25 → 100 mg) minimizes side effects and improves utilization.

Why the Sequence Matters

The protocol is designed to:

1. Prime metabolism with NAD+ (Weeks 1–2).
2. Repair mitochondria with SS‑31 once NAD+ levels are elevated (Weeks 3–8).
3. Enhance metabolic signaling with MOTS‑c after mitochondria are stabilized (Weeks 9–14).

This stepwise approach aims to maximize synergy while avoiding overlapping stressors or confounding effects.

Core Takeaway

This is a structured, research‑informed mitochondrial optimization protocol that uses NAD+ to build metabolic capacity, SS‑31 to repair mitochondrial structure and function, and MOTS‑c to enhance metabolic signaling and resilience—each introduced at the moment when the body is most prepared to benefit.

Realistic Timeline for Mitochondrial Peptides

The benefits are cumulative. You’re rebuilding cellular infrastructure.

Weeks 1-2: Subtle improvements in energy consistency. Possibly better sleep quality. Training feels marginally easier.

Weeks 3-4: Energy more stable throughout the day. Recovery windows noticeably shorter. Mental stamina improving.

Weeks 5-6: Clear difference in training capacity. Afternoon crashes mostly gone. Overall sense of vitality increasing.

Weeks 8-12: Full cumulative benefits realized. Recovery significantly faster. Body composition improvements with proper diet and training.

The key is consistency. These aren’t peptides you run for two weeks and expect dramatic results.